Two protein kinase-inhibitors, 6-dimethyl amino purine and 2-amino purine inhibited induction of β-casein synthesis by prolactin when added to the culture medium of rabbit mammary explant and cells. The accumulation of the mRNA for αs1- and β-caseins and for whey acidic protein did not take place in the presence of the inhibitors whereas β-actin mRNA concentration was not altered. In the same experimental conditions, H7, an inhibitor of protein kinase C and, to a lower extent, of protein kinase A did not prevent prolactin from acting. These data suggest for the first time that specific protein kinases are involved in the transduction of the prolactin signal to milk protein genes.
6-Dimethyl amino purine and 2-amino purine inhibit the induction of expresion of milk protein genes by prolactin
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Mahasti Bayat-Sarmadi, Rachel Maliénou-Ngassa, Claudine Puissant, Louis-Marie Houdebine; 6-Dimethyl amino purine and 2-amino purine inhibit the induction of expresion of milk protein genes by prolactin. Biosci Rep 1 June 1992; 12 (3): 189–197. doi: https://doi.org/10.1007/BF01121788
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