To study the effect of diabetes on hepatic protein synthesis and polysomal aggregation in pregnant rats, female rats were treated with streptozotocin prior to conception. Some animals were mated, and studied at day 20 of pregnancy, whereas, others were studied in parallel under non pregnant conditions. The protein synthesis rate measured with an “in vitro” cell-free system was higher in pregnant than in virgin control rats. It decreased with diabetes in both groups, although values remained higher in diabetic pregnant rats than in the virgin animals. The fetuses of diabetic rats had a lower protein synthesis rate than those from controls, although they showed a higher protein synthesis rate than either their respective mothers or virgin rats. Liver RNA concentration was higher in control and diabetic, pregnant rats than in virgin rats, and the effect of diabetes decreasing this parameter was only significant for pregnant rats. Liver RNA concentration in fetuses was lower than in their mothers, and did not differ between control and diabetic animals. The decreased protein synthesis found in diabetic animals was accompanied by disaggregation of heavy polysomes into lighter species, indicating an impairment in peptide-chain initiation.
Skip Nav Destination
Article navigation
Research Article|
February 01 1995
Effect of streptozotocin diabetes on polysomal aggregation and protein synthesis rate in the liver of pregnant rats and their offspring
M. E. Martin;
M. E. Martin
2Servicio de Bioquímica, Dpto. Investigatión, Hospital Ramón y Cajal, Ctra. Colmenar Km. 9, 28034 Madrid, Spain
1Centro de Ciencias Experimentales y Técnicas, Universidad de San Pablo-CEU, Madrid, Spain
Search for other works by this author on:
A. M. Garcia;
A. M. Garcia
2Servicio de Bioquímica, Dpto. Investigatión, Hospital Ramón y Cajal, Ctra. Colmenar Km. 9, 28034 Madrid, Spain
1Centro de Ciencias Experimentales y Técnicas, Universidad de San Pablo-CEU, Madrid, Spain
Search for other works by this author on:
L. Blanco;
L. Blanco
2Servicio de Bioquímica, Dpto. Investigatión, Hospital Ramón y Cajal, Ctra. Colmenar Km. 9, 28034 Madrid, Spain
1Centro de Ciencias Experimentales y Técnicas, Universidad de San Pablo-CEU, Madrid, Spain
Search for other works by this author on:
E. Herrera;
E. Herrera
2Servicio de Bioquímica, Dpto. Investigatión, Hospital Ramón y Cajal, Ctra. Colmenar Km. 9, 28034 Madrid, Spain
1Centro de Ciencias Experimentales y Técnicas, Universidad de San Pablo-CEU, Madrid, Spain
Search for other works by this author on:
M. Salinas
M. Salinas
2Servicio de Bioquímica, Dpto. Investigatión, Hospital Ramón y Cajal, Ctra. Colmenar Km. 9, 28034 Madrid, Spain
1Centro de Ciencias Experimentales y Técnicas, Universidad de San Pablo-CEU, Madrid, Spain
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
December 28 1994
Accepted:
January 16 1995
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1995 Plenum Publishing Corporation
1995
Biosci Rep (1995) 15 (1): 15–20.
Article history
Received:
December 28 1994
Accepted:
January 16 1995
Citation
M. E. Martin, A. M. Garcia, L. Blanco, E. Herrera, M. Salinas; Effect of streptozotocin diabetes on polysomal aggregation and protein synthesis rate in the liver of pregnant rats and their offspring. Biosci Rep 1 February 1995; 15 (1): 15–20. doi: https://doi.org/10.1007/BF01200211
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |