We examined the possibility of quantitative differences in lactate entry into periportal and perivenous hepatocytes under different nutritional states. The rate of14C-L(+)-lactate uptake was determined after 15-second incubations with freshly isolated zonally separated hepatocytes using a centrifuge stop technique at 37 °C and 4 °C, in the presence or absence of either differing amounts of unlabelled lactate or of a hepatocyte lactate transport inhibitor,α-cyano-3-hydroxycinnamate. Total entry as well as carrier mediated entry of14C-L(+)-lactate into the isolated cell populations was found to be similar in periportal and perivenous hepatocytes, irrespective of the nutritional state of the animal. Periportal and perivenous hepatocytes showed a greater tendency to transport lactate when isolated from starved animals, in agreement with previously reported data from non-zonally separated isolated hepatocytes. The activity of the hepatocyte plasma-membrane lactate transporter was diminished between fourfold and eightfold in transport studies conducted at 4 °C; similar results were obtained in unseparated and zonally separated suspensions. Temperature dependence of the hepatocyte transporter is markedly less than that reported for the erythrocyte transporter.
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Research Article|
April 01 1995
Carrier-mediated lactate entry into isolated hepatocytes from fed and starved rats: Zonal distribution and temperature dependence
M. A. Staricoff;
M. A. Staricoff
2University of Bristol, Department of Biochemistry, School of Medical Sciences, University Walk, Bristol BS8 1TD, U.K.
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R. D. Cohen;
R. D. Cohen
1Cellular Mechanisms Research Group, The London Hospital Medical College, University of London, U.K.
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J. P. Monson
J. P. Monson
1Cellular Mechanisms Research Group, The London Hospital Medical College, University of London, U.K.
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Publisher: Portland Press Ltd
Received:
October 29 1994
Accepted:
December 20 1994
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1995 Plenum Publishing Corporation
1995
Biosci Rep (1995) 15 (2): 99–109.
Article history
Received:
October 29 1994
Accepted:
December 20 1994
Citation
M. A. Staricoff, R. D. Cohen, J. P. Monson; Carrier-mediated lactate entry into isolated hepatocytes from fed and starved rats: Zonal distribution and temperature dependence. Biosci Rep 1 April 1995; 15 (2): 99–109. doi: https://doi.org/10.1007/BF01200144
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