Gamma- and delta-isomers of hexachlorocyclohexane caused marked decreases in the levels of radioactive phospholipids, and increases in the levels of [3H]arachidonate incorporated into free fatty acids in rat renal tubular cells. The increased radioactivity of free fatty acids arises from the decrease of [3H]arachidonate incorporated into phosphatidylinositol, but not into phosphatidylcholine, phosphatidylserine or phosphatidylethanolamine. This fact suggests that phosphatidylinositol can be broken down to the fatty acid from the sn-2 position and lysophospholipid by a phospholipase activity increased by hexachlorocyclohexanes. The observed specific toxicant action could be achieved in two ways: (a) operating upon a specific phospholipase A2 that acts on phosphatidylinositol, but not on other phospholipids as substrates and/or (b) involving substrate-phospholipase A2 interactions. Interestingly, the observed effect of the γ-isomer was more pronounced than that of the γ-one.
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August 01 1995
Hexachlorocyclohexanes affect the arachidonic acid release from phosphatidylinositol but not from other phospholipid classes in tubular cell cultures
J. C. Puente-Fraga;
J. C. Puente-Fraga
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, 28871 Alcala de Henares-Madrid, Spain
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P. López-Aparicio;
P. López-Aparicio
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, 28871 Alcala de Henares-Madrid, Spain
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S. Senar;
S. Senar
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, 28871 Alcala de Henares-Madrid, Spain
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M. N. Recio;
M. N. Recio
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, 28871 Alcala de Henares-Madrid, Spain
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M. A. Pérez-Albarsanz
M. A. Pérez-Albarsanz
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá de Henares, 28871 Alcala de Henares-Madrid, Spain
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Publisher: Portland Press Ltd
Received:
April 10 1995
Accepted:
July 26 1995
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1995 Plenum Publishing Corporation
1995
Biosci Rep (1995) 15 (4): 191–199.
Article history
Received:
April 10 1995
Accepted:
July 26 1995
Citation
J. C. Puente-Fraga, P. López-Aparicio, S. Senar, M. N. Recio, M. A. Pérez-Albarsanz; Hexachlorocyclohexanes affect the arachidonic acid release from phosphatidylinositol but not from other phospholipid classes in tubular cell cultures. Biosci Rep 1 August 1995; 15 (4): 191–199. doi: https://doi.org/10.1007/BF01540453
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