Proton-coupled lactate transport across the basolateral membrane of rat jejunal enterocyte was studied using well purified membrane vesicles. L-lactate uptake is stimulated by an inwardly directed H+ gradient; the effect of the pH difference is drastically reduced by FCCP and by pCMBS; unlabelled L-lactate causes a strong inhibition, whilst furosemide is uneffective. The H+ gradient-dependent stimulation of L-lactate uptake is significantly inhibited also by SCN−: this finding could explain results recently reported in the literature in which H+-lactate symport was not evidenced in basolateral membranes from rat jejunum.
Proton-lactate co transport in basolateral membrane vesicles from rat jejunum
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Maria Novella Orsenigo, Marisa Tosco, Umberto Laforenza, Alide Faelli; Proton-lactate co transport in basolateral membrane vesicles from rat jejunum. Biosci Rep 1 December 1996; 16 (6): 521–527. doi: https://doi.org/10.1007/BF01198466
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