The unicellular Tetrahymena enzymatically split the synthetic phosphodiester, 4-methylum-belliferyl phosphocoline substrate. The enzyme activity was completely blocked in vitro and drastically inhibited in vivo by G-protein activating fluorides (NaF; AlF4– and BeF3–). The phospholipase A2 inhibitor, quinacrine, and the protein phosphatase inhibitor, neomycin, inhibited the enzyme activity in vitro and activated it in vivo. Another phospholipase A2 inhibitor 4-bromo phenacyl bromide was ineffective in vivo and in vitro alike, as well as the cyclooxygenase inhibitor indomethacin. Results of these experiments indicate that some treatments could be specific for a well defined activity (e.g., phospholipase A2, G-protein) but subject to influence by other enzymes (e.g., phospholipase C, sphingomyelinase). The experiments call attention to the differences in the results of the in vivo and in vitro studies.
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Research Article|
April 01 1999
Fluorimetric Analysis of Phospholipase Activity in Tetrahymena pyriformis GL.
Péter Kovács
;
Péter Kovács
1
Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, H-1445, Budapest, POB 370, Hungary
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György Csaba
György Csaba
1
Department of Genetics, Cell and Immunobiology, Semmelweis University of Medicine, H-1445, Budapest, POB 370, Hungary
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Biosci Rep (1999) 19 (2): 81-87.
Citation
Péter Kovács, György Csaba; Fluorimetric Analysis of Phospholipase Activity in Tetrahymena pyriformis GL.. Biosci Rep 1 April 1999; 19 (2): 81–87. doi: https://doi.org/10.1023/A:1020154325630
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