Two types of rat mannose-binding proteins (MBPs), MBP-A (serum type) and MBP-C (liver type), have similar binding specificity for monosaccharide and similar binding site construct according to the X-ray structure, but exhibit different affinity toward natural oligosaccharides and glycoproteins. To understand the basis for this phenomenon, we used cloned fragment of MBP-A and -C (entire carbohydrate-recognition domain and a short connecting piece) that exists as stable trimers in various binding studies. Binding of a number of mannose-containing di- and tri-saccharides and high-mannose type oligosaccharides indicated that MBP-C has an extended binding area of weak interaction with the second and the third mannose residues, whereas MBP-A recognizes just a single mannose residue. In addition, MBP-C has a weak secondary binding site some 25 Å away from the primary site. These findings explain the higher affinity of MBP-C for natural high-mannose type oligosaccharides as compared to MBP-A. A huge affinity differential manifested by natural glycoproteins (e.g., inhibitory potency of thyroglobulin is ∼200 fold higher for MBP-C than for MBP-A in a solid-phase assay) may be due to steric hindrance experienced by MBP-A in the competition assay, and suggests different arrangement of subunit in the MBP trimers.
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Research Article|
August 01 1999
Lectin-Carbohydrate Interactions: Fine Specificity Difference Between Two Mannose-Binding Proteins
Reiko T. Lee;
Reiko T. Lee
1Department of Biology, The Johns Hopkins University, Baltimore, MD 21218 USA;
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Yasuro Shinohara;
Yasuro Shinohara
2Department of R&D, Amersham Pharmacia Biotech KK, 4-5-37 Kami-Ohsaki, Shinagawa-ku, Tokyo, 141 Japan
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Yukio Hasegawa;
Yukio Hasegawa
2Department of R&D, Amersham Pharmacia Biotech KK, 4-5-37 Kami-Ohsaki, Shinagawa-ku, Tokyo, 141 Japan
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Yuan C. Lee
Yuan C. Lee
1Department of Biology, The Johns Hopkins University, Baltimore, MD 21218 USA;
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1999 Plenum Publishing Corporation
1999
Biosci Rep (1999) 19 (4): 283–292.
Citation
Reiko T. Lee, Yasuro Shinohara, Yukio Hasegawa, Yuan C. Lee; Lectin-Carbohydrate Interactions: Fine Specificity Difference Between Two Mannose-Binding Proteins. Biosci Rep 1 August 1999; 19 (4): 283–292. doi: https://doi.org/10.1023/A:1020546307825
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