UDA (Urtica dioica agglutinin) contains two hevein like domains with two non-identical interacting sites and is specific for chitooligosaccharides. The binding of chitooligosaccharides to UDA was studied by Isothermal Titration Calorimetry. Each site is composed of three subsites, each binding to a sugar residue. Thermodynamic parameters obtained show that while chitobiose has two independent non-interacting sites, chitotriose, chitotetrose and chitopentose have two interacting sites on each monomer of UDA. Values of binding enthalpy (ΔH) increase almost by a factor of 7 in going from chitobiose to chitotriose indicating the existence of three subsites in the combining site of UDA. The binding constant for chitotetrose and chitopentose increase without any further enhancement in the values of ΔH indicating that for oligomers larger than chitotriose interaction is favoured entropically.
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Research Article|
October 01 1999
Thermodynamic Analysis of Chitooligosaccharide Binding to Urtica dioica agglutinin by Isothermal Titration Calorimetry
Samiksha Katiyar;
Samiksha Katiyar
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore-560 012
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E. J. M. Van Damme;
E. J. M. Van Damme
2Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, W. de Croylaan 42, B-3001 Heverleee, Belgium
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Willy J. Peumans;
Willy J. Peumans
2Laboratory for Phytopathology and Plant Protection, Katholieke Universiteit Leuven, W. de Croylaan 42, B-3001 Heverleee, Belgium
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Avadhesha Surolia
Avadhesha Surolia
1Molecular Biophysics Unit, Indian Institute of Science, Bangalore-560 012
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1999 Plenum Publishing Corporation
1999
Biosci Rep (1999) 19 (5): 411–419.
Citation
Samiksha Katiyar, E. J. M. Van Damme, Willy J. Peumans, Avadhesha Surolia; Thermodynamic Analysis of Chitooligosaccharide Binding to Urtica dioica agglutinin by Isothermal Titration Calorimetry. Biosci Rep 1 October 1999; 19 (5): 411–419. doi: https://doi.org/10.1023/A:1020264206433
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