Human polymorphonuclear leukocytes (PMN) were found to tightly adhere on endothelial (lines EAhy926 and ECV304) and collagen surfaces under the influence of the chemotherapeutic drug suramin. This was observed by scanning electron microscopy and quantitated by myeloperoxidase assays. Suramin also inhibited Ca2+ ionophore A23187-stimulated leukotriene (LT) synthesis in PMN interaction with endothelial cells or with collagen surface. Suramin decreased the release of radiolabeled arachidonic acid (AA) and 5-lip-oxygenase (5-LO) metabolites by prelabeled PMN stimulated with A23187. Using agents releasing the suramin-stimulated adhesion namely jasplakonolide and dextran sulfate, we observed a reversal of the suramin effect on leukotriene synthesis. Jasplakonolide released the adhesion of PMN on endothelial and collagen-coated surfaces and restored 5-LO activity. Dextran-sulfate released adhesion on collagen-coated surfaces and abolished suramin inhibition. Arachidonate could also overcome adhesion and inhibition of 5-LO. We conclude that suramin-induced tight attachment of PMN on to solid surfaces lead to decreased leukotriene synthesis during subsequent A23187 stimulation in the absence of exogenous substrates.
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December 01 1999
Effects of Suramin on PMN Interactions with Different Surfaces
Galina F. Sud'ina;
Galina F. Sud'ina
1A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia
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Marina A. Pushkareva;
Marina A. Pushkareva
1A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia
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Svetlana I. Galkina;
Svetlana I. Galkina
1A. N. Belozersky Institute of Physico-Chemical Biology, Moscow State University, Moscow 119899, Russia
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Sergey A. Surkov;
Sergey A. Surkov
2V. A. Engelhardt Institute of Molecular Biology of the Russian Academy of Sciences, Moscow 117984, Russia
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Martin Mehl;
Martin Mehl
3Faculty of Biology, University of Konstanz, 78457 Konstanz, Germany
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Volker Ullrich
Volker Ullrich
3Faculty of Biology, University of Konstanz, 78457 Konstanz, Germany
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1999 Plenum Publishing Corporation
1999
Biosci Rep (1999) 19 (6): 547–558.
Citation
Galina F. Sud'ina, Marina A. Pushkareva, Svetlana I. Galkina, Sergey A. Surkov, Martin Mehl, Volker Ullrich; Effects of Suramin on PMN Interactions with Different Surfaces. Biosci Rep 1 December 1999; 19 (6): 547–558. doi: https://doi.org/10.1023/A:1020214928298
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