The trafficking of intracellular membrane proteins in Golgi apparatus, endoplasmic reticulum or intermediate compartment has not yet been fully elucidated. The human MR60/ERGIC-53 and the rat p58 proteins are one such protein; and to study them in cell-free and in situ systems, high quality monospecific antisera are required. Highly specific antisera have been obtained after immunization of mice with plasmids containing a gene encoding either the full length or a truncated protein. The best results were obtained after intradermal injections of a plasmid encoding a truncated protein comprising both the luminal carbohydrate recognition domain and the stem down to a cysteine residue close to the C-terminal end, but neither the transmembrane nor the cytosolic domains. Such antisera have a very high titer and are very efficient tools to visualize the MR60 protein in situ or to selectively precipitate the MR60 proteins from a whole cell lysate.
Intradermal DNA Immunization: Antisera Specific for the Membrane Lectin MR60/ERGIC-53
- Views Icon Views
- Share Icon Share
- Cite Icon Cite
Violaine Carrière, Ludovic Landemarre, Valérie Altemayer, Geneviève Motta, Michel Monsigny, Annie-Claude Roche; Intradermal DNA Immunization: Antisera Specific for the Membrane Lectin MR60/ERGIC-53. Biosci Rep 1 December 1999; 19 (6): 559–569. doi: https://doi.org/10.1023/A:1020266912368
Download citation file: