Transgene expression in lymphoid cells may be useful for modulating immune responses in, and gene therapy of, cancer and AIDS. Although cationic liposome-DNA complexes (lipoplexes) present advantages over viral vectors, they have low transfection efficiency, unfavorable features for intravenous administration, and lack of target cell specificity. The use of a targeting ligand (transferrin), or an endosome-disrupting peptide, in ternary complexes with liposomes and a luciferase plasmid, significantly promoted transgene expression in several T- and B-lymphocytic cell lines. The highest levels of luciferase activity were obtained at a lipid/DNA (±) charge ratio of 1/1, where the ternary complexes were net negatively charged. The use of such negatively charged ternary complexes may alleviate some of the drawbacks of highly positively charged plain lipoplexes for gene delivery.
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Research Article|
December 01 1999
Successful Transfection of Lymphocytes by Ternary Lipoplexes
S. Simões;
S. Simões
1Department of Microbilogy, School of Dentistry, University of the Pacific, San Francisco, CA, 94115, USA
2Laboratory of Pharmaceutical Technology, Faculty of Pharmacy. Center for Neurosciences, USA
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V. Slepushkin;
V. Slepushkin
1Department of Microbilogy, School of Dentistry, University of the Pacific, San Francisco, CA, 94115, USA
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R. Gaspar;
R. Gaspar
2Laboratory of Pharmaceutical Technology, Faculty of Pharmacy. Center for Neurosciences, USA
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M. C. Pedroso de Lima;
M. C. Pedroso de Lima
2Laboratory of Pharmaceutical Technology, Faculty of Pharmacy. Center for Neurosciences, USA
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N. Düzgünes
N. Düzgünes
1Department of Microbilogy, School of Dentistry, University of the Pacific, San Francisco, CA, 94115, USA
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1999 Plenum Publishing Corporation
1999
Biosci Rep (1999) 19 (6): 601–609.
Citation
S. Simões, V. Slepushkin, R. Gaspar, M. C. Pedroso de Lima, N. Düzgünes; Successful Transfection of Lymphocytes by Ternary Lipoplexes. Biosci Rep 1 December 1999; 19 (6): 601–609. doi: https://doi.org/10.1023/A:1020275114185
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