Reduction of the α-isoprene unit of polyprenols to form dolichols was studied in vivo using 3H-polyprenol derivatives as substrates and liposomes as carriers. Liposomes containing labeled polyprenol, polyprenyl phosphate, or polyprenyl pyrophosphate were injected through the portal vein into the livers of rats under anesthesia. Uptake and conversion of the labeled compounds to dolichol derivatives was studied at different intervals. The greatest conversion to dolichol derivatives was found with polyprenyl pyrophosphate and polyprenyl monophosphate, with 31% and 8% of the absorbed dose converted respectively. Less than 0.2% of the absorbed polyprenol was converted to dolichol derivatives. These results suggest that the substrate for the α-isoprene reductase involved in dolichol biosynthesis is either polyprenyl monophosphate or polyprenyl pyrophosphate, or both.
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Research Article|
October 01 1982
In vivo biosynthesis of the saturated isoprene unit of dolichyl phosphate
Carmen Vigo;
Carmen Vigo
1Department of Biochemistry, University of South Florida College of Medicine, Tampa, FL 33612, U.S.A.
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W. Lee Adair, Jr.
W. Lee Adair, Jr.
1Department of Biochemistry, University of South Florida College of Medicine, Tampa, FL 33612, U.S.A.
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Publisher: Portland Press Ltd
Received:
September 02 1982
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1982 The Biochemical Society
1982
Biosci Rep (1982) 2 (10): 835–840.
Article history
Received:
September 02 1982
Citation
Carmen Vigo, W. Lee Adair; In vivo biosynthesis of the saturated isoprene unit of dolichyl phosphate. Biosci Rep 1 October 1982; 2 (10): 835–840. doi: https://doi.org/10.1007/BF01114944
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