The distribution, synthesis transport, and glycosylation of rat-liver DT-diaphorase has been investigated. The enzyme could be isolated using specific antibodies, mainly from the soluble supernatant but also from microsomal vesicles, Golgi membrane, and mitochondria. 40% of the microsomal enzyme was located in the lumen or on the interior side of the membrane, the rest remaining as an integral non-extractable part of the membrane. Synthesis of DT-diaphorase takes place on both free and bound ribosomes, although it was found to be transported in a sequential manner from the rough to the smooth endoplasmic reticulum and also subsequently to the mitochondria. The rough and smooth microsomal DT-diaphorase contains covalently bound carbohydrate, but no sugar moiety could be detected bound to the cytoplasmic form of the enzyme.
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November 01 1982
Distribution of newly synthesized DT-diaphorase in rat liver
C. Edlund;
C. Edlund
*Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm
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Å. Elhammer;
Å. Elhammer
*Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm
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G. Dallner
G. Dallner
*Department of Biochemistry, Arrhenius Laboratory, University of Stockholm, S-106 91, Stockholm
†Department of Pathology, Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden
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Publisher: Portland Press Ltd
Received:
September 13 1982
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1982 The Biochemical Society
1982
Biosci Rep (1982) 2 (11): 861–865.
Article history
Received:
September 13 1982
Citation
C. Edlund, Å. Elhammer, G. Dallner; Distribution of newly synthesized DT-diaphorase in rat liver. Biosci Rep 1 November 1982; 2 (11): 861–865. doi: https://doi.org/10.1007/BF01114891
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