The distribution, synthesis transport, and glycosylation of rat-liver DT-diaphorase has been investigated. The enzyme could be isolated using specific antibodies, mainly from the soluble supernatant but also from microsomal vesicles, Golgi membrane, and mitochondria. 40% of the microsomal enzyme was located in the lumen or on the interior side of the membrane, the rest remaining as an integral non-extractable part of the membrane. Synthesis of DT-diaphorase takes place on both free and bound ribosomes, although it was found to be transported in a sequential manner from the rough to the smooth endoplasmic reticulum and also subsequently to the mitochondria. The rough and smooth microsomal DT-diaphorase contains covalently bound carbohydrate, but no sugar moiety could be detected bound to the cytoplasmic form of the enzyme.
Research Article| November 01 1982
Distribution of newly synthesized DT-diaphorase in rat liver
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C. Edlund, Å. Elhammer, G. Dallner; Distribution of newly synthesized DT-diaphorase in rat liver. Biosci Rep 1 November 1982; 2 (11): 861–865. doi: https://doi.org/10.1007/BF01114891
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