The dideoxy sequencing technique has been applied to the direct sequencing of large double-stranded DNA molecules with a small single-stranded primer. For instance, the method was applied to the lambda genome, which contains 48 502 base-pairs (Sanger F, Coulson AR, Hong GF, Hill D & Petersen GB, 1982, J. Mol. Biol., in press), and the coding region for gene W identified. The procedure proves useful in the sequence analysis of a large number of different mutations in a particular region and in the analysis of eukaryotic DNA cloned in plasmids, phages, and cosmids.
Research Article| November 01 1982
Sequencing of large double-stranded DNA using the dideoxy sequencing technique
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G. F. Hong; Sequencing of large double-stranded DNA using the dideoxy sequencing technique. Biosci Rep 1 November 1982; 2 (11): 907–912. doi: https://doi.org/10.1007/BF01114897
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