The kinetics of Pr3+ transport by the ionophores alamethicin 30 and A23187 across unilamellar phospho-lipid vesicular membranes has been compared by following the time-dependent changes in the 1H-NMR spectrum of the vesicles. The measured rates of transport allow stoichiometries of the transporting species to be deduced which are consistent with channel- and carrier-mediated mechanisms respectively. The method provides a useful complement to planar bilayer conductivity studies of these systems.
Lanthanide-ion transport across phospholipid vesicular membranes: a comparison of alamethicin 30 and A23187 using 1H-NMR spectroscopy
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G. R. A. Hunt, I. C. Jones; Lanthanide-ion transport across phospholipid vesicular membranes: a comparison of alamethicin 30 and A23187 using 1H-NMR spectroscopy. Biosci Rep 1 November 1982; 2 (11): 921–928. doi: https://doi.org/10.1007/BF01114899
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