Rat-liver nucleoli (10–15 pg DNA) were digested with either 0.6 or 3 units of DNase I for various times (up to 1 h). RNA synthesis was then measured in the absence or presence ol 3 units of Escherichia coli RNA polymerase. It was found that the nucleolar chromatin supporting the endogenous engaged RNA polymerase I transcription was compl-etely destroyed in 3 min with either concentration of DNase I. The nucleolar chromatin template transcribed by E. coli RNA polymerase retained 50% of its original capacity even 60 min alter 3 units of DNase I digestion. When hybridization experiments were conducted, it was found that the DNAs derived from both levels of DNase-Idigested nucleoli were incapable of forming hybrids with the labelled nucleolar RNA synthesized by the engaged RNA polymerase I from the untreated nucleoli. Since the engaged RNA polymerase I transcribes only the physiologically active genes of the nucleolar chromatin, and the RNA transcripts represent active gene product, these data suggest that DNase I digestion has completely destroyed the active genes of the nucleolar chromatin, and E. coli RNA polymerase is able to transcribe the inactive nucleolar chromatin template.
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Research Article|
March 01 1982
Evidence for the transcription of physiologically inactive rat-liver nucleolar chromatin by Escherichia coli RNA polymerase
Fu-Li Yu;
Fu-Li Yu
1Department of Biomedical Sciences, Rockford School of Medicine, University of Illinois College of Medicine, 1601 Parkview Avenue, Rockford, IL 61107, U.S.A.
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Annabella Barrett
Annabella Barrett
1Department of Biomedical Sciences, Rockford School of Medicine, University of Illinois College of Medicine, 1601 Parkview Avenue, Rockford, IL 61107, U.S.A.
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Publisher: Portland Press Ltd
Received:
February 08 1982
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1982 The Biochemical Society
1982
Biosci Rep (1982) 2 (3): 155–161.
Article history
Received:
February 08 1982
Citation
Fu-Li Yu, Annabella Barrett; Evidence for the transcription of physiologically inactive rat-liver nucleolar chromatin by Escherichia coli RNA polymerase. Biosci Rep 1 March 1982; 2 (3): 155–161. doi: https://doi.org/10.1007/BF01116378
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