The role of the topoisomerase enzyme in DNA recombination was investigated by extracting chromosomal deoxyribonucleoproteins from a variety of cultured mammalian ceils and assaying for the formation of recombinant DNA structures. Although each of the crude deoxyribonucleoprotein preparations contained topoisomerase activity, they did not all contain DNA-recombining activity. A distinct, perhaps novel, enzyme may therefore promote DNA recombination in these cell-free systems.
Mammalian cells contain a DNA-recombining activity that can be dissociated from topoisomerase activity
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C. Hamelin, R. Alain, D. Houde, L. Berthiaume; Mammalian cells contain a DNA-recombining activity that can be dissociated from topoisomerase activity. Biosci Rep 1 July 1982; 2 (7): 527–533. doi: https://doi.org/10.1007/BF01115252
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