Human lung adenocarcinoma A549 cells sensitive and A549/DDP cells resistantto Cis-dichlorodiammine platinum[II] (cisplatin) exhibit differentintracellular free calcium and calcium fluorescence images labeled withFura-2/AM and Fluo-3/AM as judged by dual-excitation fluorescence assay,Miracal Imaging and Laser Scanning Confocal Microscopy (LSCM) of singlecells. The concentration of intracellular free calcium of the resistantA549/DDP cells is one third that of the sensitive A549 cells. The effluxof Rhodamine 123 in resistant A549/DDP cells is faster than that insensitive A549 cells. In addition, A549/DDP cells have an increase ofPhosphatidylinositol 4-kinase (PtdIns 4-kinase) activity in the plasmamembrane. So it is tentatively suggested that the increase in PtIns4-kinase activity resulting from lower intracellular Ca2+concentration leads to an increase of its enzymaticproducts”�”�PIP and PIP2, which may stimulate the activity ofP-glycoprotein.
Research Article| June 01 2000
Intracellular Free Calcium Concentration and Cisplatin Resistance in Human Lung Adenocarcinoma A549 Cells
Biosci Rep (2000) 20 (3): 129–138.
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Xingjie Liang, Youguo Huang; Intracellular Free Calcium Concentration and Cisplatin Resistance in Human Lung Adenocarcinoma A549 Cells. Biosci Rep 1 June 2000; 20 (3): 129–138. doi: https://doi.org/10.1023/A:1005530501137
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