Mouse carrier erythrocytes containing 125I-interleukin 3 have been prepared and treated with band 3 crosslinking reagents. The incorporation of interleukin 3 by hypotonic treatment into mouse erythrocytes reached levels of about 15% of the interleukin 3 added to the medium being predominantly present in the cytosolic fraction (73%). Uptake fell to about 7.4% when using the same conditions but omitting hypotonic shock. The interaction of band 3 crosslinked interleukin 3 loaded erythrocytes with macrophages was also studied. A high level of incorporation of interleukin 3 into macrophages was observed either from band 3 crosslinked, interleukin 3-loaded erythrocytes or from interleukin 3 loaded erythrocytes. The observations encourage the view that the system may be able to deliver and target cytokines and other growth factors to macrophages.
Research Article| October 01 2000
Delivery to Macrophages of Interleukin 3 Loaded in Mouse Erythrocytes
L. Alfredo Lotero;
M. Cristina Tejedor;
Biosci Rep (2000) 20 (5): 399–410.
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Gemma Olmos, L. Alfredo Lotero, M. Cristina Tejedor, José C. Diez; Delivery to Macrophages of Interleukin 3 Loaded in Mouse Erythrocytes. Biosci Rep 1 October 2000; 20 (5): 399–410. doi: https://doi.org/10.1023/A:1010334118492
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