β-mannosidase is an exoglycosidase involved in the degradation of N-linked oligosacharides moieties of glycoproteins. Lack of β-mannosidase activity leads to the lysosomal disorder β-mannosidosis (MIM 248510). We have isolated and sequenced the gene encoding the mouse β-mannosidase. Comparison of the deduced amino acid sequence of mouse, human, bovine, and goat β-mannosidase showed 64% identity, reflecting a high degree of evolutionary conservation. Analysis of a multiple tissue northern blotting revealed a major transcript of about 3.7 kb in all tissues examined. The northern analysis also demonstrates that there is differential tissue mRNA expression. The mouse β-mannosidase gene (Bmn) was mapped to the distal end of Chromosome (Chr) 3, in a region that is homologous with a segment of human Chr 4 containing the orthologous human gene.
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June 01 2001
Mouse Beta-Mannosidase: cDNA Cloning, Expression, and Chromosomal Localization
Tommaso Beccari;
Tommaso Beccari
1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Via del Giochetto, 06100 Perugia, Italy
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Lucia Bibi;
Lucia Bibi
1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Via del Giochetto, 06100 Perugia, Italy
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Sofia Stinchi;
Sofia Stinchi
1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Via del Giochetto, 06100 Perugia, Italy
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John Laing Stirling;
John Laing Stirling
2Division of Life Sciences, King's College London, 150 Stamford Street, London, UK
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Aldo Orlacchio
Aldo Orlacchio
1Dipartimento di Scienze Biochimiche e Biotecnologie Molecolari, Università degli Studi di Perugia, Via del Giochetto, 06100 Perugia, Italy
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2001 Plenum Publishing Corporation
2001
Biosci Rep (2001) 21 (3): 315–323.
Citation
Tommaso Beccari, Lucia Bibi, Sofia Stinchi, John Laing Stirling, Aldo Orlacchio; Mouse Beta-Mannosidase: cDNA Cloning, Expression, and Chromosomal Localization. Biosci Rep 1 June 2001; 21 (3): 315–323. doi: https://doi.org/10.1023/A:1013286216030
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