The connexin45 (Cx45) gene was cloned from a mouse genomic Bacterial Artificial Chromosome library. Approximately 8.4 kb of the genomic DNA was sequenced, and the structure of the Cx45 gene was determined. The mouse Cx45 gene is composed of 3 exons, with the entire coding sequence contained within exon III (EMBL Accession Number AJ300716). This structure is unique for the Cx45 gene, since all other members of the connexin family have only two exons. In addition, computer analysis reveals a potential TATA box and two putative AP-1 binding sites in the 5′ region of the gene. Sequence alignment with connexin43 indicates substantial homology in the intronic sequences upstream of the 3′ exons of the two genes, suggesting that the Cx45 gene is inherently similar to the rest of the connexin family, and that it probably evolved from an ancestor common to the other connexins.
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October 01 2001
Sequence and Structure of the Mouse Connexin45 Gene
Dustin Baldridge;
Dustin Baldridge
1Division of Bone and Mineral Diseases, Department of Internal Medicine, Washington University School of Medicine, and Barnes-Jewish Hospital, St. Louis, Missouri, USA
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Fernando Lecanda;
Fernando Lecanda
1Division of Bone and Mineral Diseases, Department of Internal Medicine, Washington University School of Medicine, and Barnes-Jewish Hospital, St. Louis, Missouri, USA
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Chan-Soo Shin;
Chan-Soo Shin
1Division of Bone and Mineral Diseases, Department of Internal Medicine, Washington University School of Medicine, and Barnes-Jewish Hospital, St. Louis, Missouri, USA
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Joseph Stains;
Joseph Stains
1Division of Bone and Mineral Diseases, Department of Internal Medicine, Washington University School of Medicine, and Barnes-Jewish Hospital, St. Louis, Missouri, USA
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Roberto Civitelli
Roberto Civitelli
1Division of Bone and Mineral Diseases, Department of Internal Medicine, Washington University School of Medicine, and Barnes-Jewish Hospital, St. Louis, Missouri, USA
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2001 Plenum Publishing Corporation
2001
Biosci Rep (2001) 21 (5): 683–689.
Citation
Dustin Baldridge, Fernando Lecanda, Chan-Soo Shin, Joseph Stains, Roberto Civitelli; Sequence and Structure of the Mouse Connexin45 Gene. Biosci Rep 1 October 2001; 21 (5): 683–689. doi: https://doi.org/10.1023/A:1014777111259
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