The effects of a commercial polychlorinated biphenyl (PCB) mixture (Aroclor 1248) and two individual PCB congeners were evaluated on rat renal proximal tubule culture cell viability and internucleosomal DNA fragmentation (DNA ladder) characteristic of apoptosis. Treatment with Aroclor 1248 caused the loss of cell viability and promoted apoptosis in a concentration- and time-dependent manner. The two PCB congeners assessed can also induce apoptosis. However, the extent of apoptosis generated was greater for the non-ortho-substituted planar congener (3,3′,4,4′-tetrachlorobiphenyl) than for the di-ortho-substituted nonplanar congener (2,2′,4,4′,5,5′-hexachlorobiphenyl). This correlated with the loss of cell viability since the planar compound is much more cytotoxic. The results suggest a different molecular mechanism in the induction of apoptosis by planar or nonplanar PCB congeners.
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December 01 2001
Different Molecular Capacity in the Induction of Apoptosis by Polychlorinated Biphenyl Congeners in Rat Renal Tubular Cell Cultures
Pedro L. Pérez-Reyes;
Pedro L. Pérez-Reyes
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain
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Jesús A. Sánchez-Alonso;
Jesús A. Sánchez-Alonso
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain
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Pilar López-Aparicio;
Pilar López-Aparicio
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain
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María N. Recio;
María N. Recio
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain
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Miguel A. Pérez-Albarsanz
Miguel A. Pérez-Albarsanz
1Departamento de Bioquímica y Biología Molecular, Universidad de Alcalá, 28871, Alcalá de Henares-Madrid, Spain
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2001 Plenum Publishing Corporation
2001
Biosci Rep (2001) 21 (6): 765–778.
Citation
Pedro L. Pérez-Reyes, Jesús A. Sánchez-Alonso, Pilar López-Aparicio, María N. Recio, Miguel A. Pérez-Albarsanz; Different Molecular Capacity in the Induction of Apoptosis by Polychlorinated Biphenyl Congeners in Rat Renal Tubular Cell Cultures. Biosci Rep 1 December 2001; 21 (6): 765–778. doi: https://doi.org/10.1023/A:1015532707395
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