Ca-ATPase activity in sarcoplasmic reticulum (SR) membranes isolated from skeletal muscles of the typical hibernator, the ground squirrel Spermophilus undulatus, is about 2-fold lower than that in SR membranes of rats and rabbits and is further decreased 2-fold during hibernation. The use of carbocyanine anionic dye Stains-All has revealed that Ca-binding proteins of SR membranes, histidine-rich Ca-binding protein and sarcalumenin, in ground squirrel, rat, and rabbit SR have different electrophoretic mobility corresponding to apparent molecular masses 165, 155, and 170 kDa and 130, 145, and 160 kDa, respectively; the electrophoretic mobility of calsequestrin (63 kDa) is the same in all preparations. The content of these Ca-binding proteins in SR membranes of the ground squirrels is decreased 3–4 fold and the content of 55, 30, and 22 kDa proteins is significantly increased during hibernation.
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December 01 2001
Ca-ATPase Activity and Protein Composition of Sarcoplasmic Reticulum Membranes Isolated from Skeletal Muscles of Typical Hibernator, the Ground Squirrel Spermophilus undulatus
Anna N. Malysheva;
Anna N. Malysheva
1Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia
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Kenneth B. Storey;
Kenneth B. Storey
2Department of Biology and Department of Chemistry, Carleton University, Ottawa K1S 5B6, Canada
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Olga D. Lopina;
Olga D. Lopina
1Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia
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Alexander M. Rubstov
Alexander M. Rubstov
1Department of Biochemistry, School of Biology, Lomonosov Moscow State University, Moscow, 119899 Russia
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Publisher: Portland Press Ltd
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 2001 Plenum Publishing Corporation
2001
Biosci Rep (2001) 21 (6): 831–838.
Citation
Anna N. Malysheva, Kenneth B. Storey, Olga D. Lopina, Alexander M. Rubstov; Ca-ATPase Activity and Protein Composition of Sarcoplasmic Reticulum Membranes Isolated from Skeletal Muscles of Typical Hibernator, the Ground Squirrel Spermophilus undulatus. Biosci Rep 1 December 2001; 21 (6): 831–838. doi: https://doi.org/10.1023/A:1015540909212
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