Chemical and physical stimuli trigger a cutaneous response by first inducing the main epidermal cells, keratinocytes, to produce specific mediators that are responsible for the initiation of skin inflammation. Activation modulates cell communication, namely leucocyte recruitment and blood-to-skin extravasation through the selective barrier of the vascular ECs (endothelial cells). In the present study, we describe an in vitro model which takes into account the various steps of human skin inflammation, from keratinocyte activation to the adhesion of leucocytes to dermal capillary ECs. Human adult keratinocytes were subjected to stress by exposure to UV irradiation or neuropeptides, then the conditioned culture medium was used to mimic the natural micro-environmental conditions for dermal ECs. A relevant in vitro model must include appropriate cells from the skin. This is shown in the present study by the selective reaction of dermal ECs compared with EC lines from distinct origins, in terms of leucocyte recruitment, sensitivity to stress and nature of the stress-induced secreted mediators. This simplified model is suitable for the screening of anti-inflammatory molecules whose activity requires the presence of various skin cells.
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Research Article|
February 08 2008
Cell model of inflammation
Jocelyne Franchi;
Jocelyne Franchi
* Laboratoires de Recherche et Développement, LVMH Branche Parfums et Cosmétiques, 45804 Saint Jean de Braye, France
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Clarisse Marteau;
Clarisse Marteau
* Laboratoires de Recherche et Développement, LVMH Branche Parfums et Cosmétiques, 45804 Saint Jean de Braye, France
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Claire Crola Da Silva;
Claire Crola Da Silva
† Centre de Biophysique Moléculaire, UPR 4301 CNRS, Université d'Orléans, INSERM, rue Ch. Sadron, 45071 Orléans, France
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Michèle Mitterrand;
Michèle Mitterrand
† Centre de Biophysique Moléculaire, UPR 4301 CNRS, Université d'Orléans, INSERM, rue Ch. Sadron, 45071 Orléans, France
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Patrice André;
Patrice André
* Laboratoires de Recherche et Développement, LVMH Branche Parfums et Cosmétiques, 45804 Saint Jean de Braye, France
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Claudine Kieda
Claudine Kieda
1
† Centre de Biophysique Moléculaire, UPR 4301 CNRS, Université d'Orléans, INSERM, rue Ch. Sadron, 45071 Orléans, France
1 To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
August 27 2007
Revision Received:
October 31 2007
Accepted:
November 07 2007
Accepted Manuscript online:
November 08 2007
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biosci Rep (2008) 28 (1): 23–32.
Article history
Received:
August 27 2007
Revision Received:
October 31 2007
Accepted:
November 07 2007
Accepted Manuscript online:
November 08 2007
Citation
Jocelyne Franchi, Clarisse Marteau, Claire Crola Da Silva, Michèle Mitterrand, Patrice André, Claudine Kieda; Cell model of inflammation. Biosci Rep 1 February 2008; 28 (1): 23–32. doi: https://doi.org/10.1042/BSR20070012
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