A novel gene, designated as GhNPR1 (Gossypium hirsutum non-expressor of pathogenesis-related genes 1), was isolated from G. hirsutum (cotton) by RT–PCR (reverse transcription–PCR) and RACE (rapid amplification of cDNA ends). The full-length cDNA was 2108 bp long and had an ORF (open reading frame) that putatively encoded a polypeptide of 592 amino acids, with a predicted molecular mass of 66 kDa. Comparison of this protein sequence with that of Arabidopsis thaliana, Brassica juncea and Nicotiana tabacum showed that the amino-acid homology was 52.98, 52.32 and 54.98% respectively. Analysis of the exon–intron structure of the GhNPR1 gene showed that GhNPR1 consisted of four exons and three introns. Southern-blot analysis revealed that the GhNPR1 was a single-copy gene in cotton. Northern-blot analysis indicated that GhNPR1 was constitutively expressed in all tested tissues, including roots, stems and leaves, with the high expression in stems and leaves. In addition, GhNPR1 was also found to be induced by signalling molecules for plant defence responses, such as methyl jasmonate, salicylic acid and ethylene, as well as attack by pathogens, such as Fusarium oxysporum and Xanthomonas campestris. These results suggest that GhNPR1 may play an important role in the response to pathogen infections in cotton plants.
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Research Article|
February 05 2008
Molecular cloning and characterization of GhNPR1, a gene implicated in pathogen responses from cotton (Gossypium hirsutum L.)
Ying Zhang;
Ying Zhang
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
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Xia Wang;
Xia Wang
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
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Cheng Cheng;
Cheng Cheng
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
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Qiuqiang Gao;
Qiuqiang Gao
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
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Jingyun Liu;
Jingyun Liu
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
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Xingqi Guo
Xingqi Guo
1
1State Key Laboratory of Crop Biology, College of Life Sciences, Shandong Agricultural University, Taian, Shandong, 271018, China
1 To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
August 06 2007
Accepted:
September 28 2007
Accepted Manuscript online:
December 18 2007
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biosci Rep (2008) 28 (1): 7–14.
Article history
Received:
August 06 2007
Accepted:
September 28 2007
Accepted Manuscript online:
December 18 2007
Citation
Ying Zhang, Xia Wang, Cheng Cheng, Qiuqiang Gao, Jingyun Liu, Xingqi Guo; Molecular cloning and characterization of GhNPR1, a gene implicated in pathogen responses from cotton (Gossypium hirsutum L.). Biosci Rep 1 February 2008; 28 (1): 7–14. doi: https://doi.org/10.1042/BSR20070028
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