An analysis of the primary structure of the actin-binding protein fesselin revealed it to be the avian homologue of mammalian synaptopodin 2 [Schroeter, Beall, Heid, and Chalovich (2008) Biochem. Biophys. Res. Commun. 371, 582–586]. We isolated two synaptopodin 2 isoforms from rabbit stomach that corresponded to known types of human synaptopodin 2. The purification scheme used was that developed for avian fesselin. These synaptopodin 2 forms shared several key functions with fesselin. Both avian fesselin and mammalian synaptopodin 2 bound to Ca2+–calmodulin, α-actinin and smooth-muscle myosin. In addition, both proteins stimulated the polymerization of actin in a Ca2+–calmodulin-dependent manner. Synaptopodin 2 has never before been shown to polymerize actin in the absence of α-actinin, to polymerize actin in a Ca2+–calmodulin-dependent manner, or to bind to Ca2+–calmodulin or myosin. These properties are consistent with the proposed function of synaptopodin 2 in organizing the cytoskeleton.
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Research Article|
September 01 2008
In vitro characterization of native mammalian smooth-muscle protein synaptopodin 2
Mechthild M. Schroeter;
Mechthild M. Schroeter
1
*Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, U.S.A.
1To whom correspondence should be addressed (e-mail [email protected]).
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Brent Beall;
Brent Beall
*Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, U.S.A.
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Hans W. Heid;
Hans W. Heid
†German Cancer Research Center, Cell Biology, D-69120 Heidelberg, Germany
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Joseph M. Chalovich
Joseph M. Chalovich
*Department of Biochemistry and Molecular Biology, Brody School of Medicine at East Carolina University, Greenville, NC 27834, U.S.A.
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Publisher: Portland Press Ltd
Received:
June 23 2008
Accepted:
June 30 2008
Accepted Manuscript online:
June 30 2008
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biosci Rep (2008) 28 (4): 195–203.
Article history
Received:
June 23 2008
Accepted:
June 30 2008
Accepted Manuscript online:
June 30 2008
Citation
Mechthild M. Schroeter, Brent Beall, Hans W. Heid, Joseph M. Chalovich; In vitro characterization of native mammalian smooth-muscle protein synaptopodin 2. Biosci Rep 1 August 2008; 28 (4): 195–203. doi: https://doi.org/10.1042/BSR20080079
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