AOC (allene oxide cyclase; EC 5.3.99.6), an essential enzyme in jasmonic acid and its methyl ester biosynthesis, was cloned from Camptotheca acuminata (named as CaAOC), a native medicinal plant species in China. CaAOC had significant similarity at the amino-acid level with AOCs from other plant species. Comparison between the sequences of the full-length cDNA and genomic DNA of CaAOC revealed that the genomic DNA of CaAOC contained an 89-bp intron and a 240-bp intron. Southern-blot analysis indicated that CaAOC was a multiple-copy gene, and real-time quantitative PCR analysis showed that CaAOC was expressed constitutively in all organs tested, with the highest expression level in leaves. The results from treatment experiments using different signalling components, including methyl jasmonate, abscisic acid, salicylic acid and H2O2, revealed that expression of CaAOC had a prominent diversity. Heavy metal (copper) significantly enhanced CaAOC expression, whereas wounding (induced by UV-B) was not so effective.
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November 13 2008
Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata
Yan Pi;
Yan Pi
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Zhihua Liao;
Zhihua Liao
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
†Laboratory of Natural Products and Metabolic Engineering, Institute of Biotechnology, School of Life Sciences, Southwest University, Chongqing 400715, People's Republic of China
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Keji Jiang;
Keji Jiang
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Beibei Huang;
Beibei Huang
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Zhongxiang Deng;
Zhongxiang Deng
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Dongli Zhao;
Dongli Zhao
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Hainian Zeng;
Hainian Zeng
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Xiaofen Sun;
Xiaofen Sun
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
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Kexuan Tang
Kexuan Tang
1
*State Key Laboratory of Genetic Engineering, School of Life Sciences, Morgan-Tan International Center for Life Sciences, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, Fudan University, Shanghai 200433, People's Republic of China
‡Plant Biotechnology Research Center, Fudan–SJTU–Nottingham Plant Biotechnology R&D Center, School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200030, People's Republic of China
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
May 30 2006
Accepted:
December 06 2006
Accepted Manuscript online:
October 13 2008
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2008 Biochemical Society
2008
Biosci Rep (2008) 28 (6): 349–355.
Article history
Received:
May 30 2006
Accepted:
December 06 2006
Accepted Manuscript online:
October 13 2008
Citation
Yan Pi, Zhihua Liao, Keji Jiang, Beibei Huang, Zhongxiang Deng, Dongli Zhao, Hainian Zeng, Xiaofen Sun, Kexuan Tang; Molecular cloning, characterization and expression of a jasmonate biosynthetic pathway gene encoding allene oxide cyclase from Camptotheca acuminata. Biosci Rep 1 December 2008; 28 (6): 349–355. doi: https://doi.org/10.1042/BSR20060001
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