Quantitative analysis of the phospholipid composition of pulmonary surfactant preparations has been achieved by a modification of the phosphorus-31 nuclear magnetic resonance method of E London and G Feigenson (3. Lipid Res. 20, 408–412, 1979). Resolution of the protein components by a 2-dimensional isoelectric-focussing - SDS/ polyacrylamide-gel-electrophoresis technique is reported for the first time.

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