The thermo-responsivity of PNIPAAm [poly(N-isopropylcarylamide)]-grafted PDMS [poly(dimethylsiloxane)] surface is a property that could be feasibly used for detaching cells adhered on the surface. We used benzophenone-initiated photopolymerization to graft PNIPAAm on PDMS substrates to construct the PNIPAAm-grafted PDMS surface and this PDMS surface was highly thermo-responsive. hMSCs (human mesenchymal stem cells) were used to analyse the proliferation and multi-differentiation of stem cells on the PNIPAAm-grafted PDMS surface. The results showed that hMSCs could adhere on the PNIPAAm-grafted PDMS surface at 37°C and form cell colonies, and then become fibroblastic. The proliferation potential of hMSCs on the PNIPAAm-grafted PDMS surface was not significantly different from that on a plate surface coated with gelatin. However, as it proved easier to detach cells from the surface, by changing temperature, a higher viability of detached cells could be obtained with the PNIPAAm-grafted PDMS surface, using a temperature shift, compared with a gelatin-coated surface, where cells are detached by treatment with trypsin. hMSCs on the PNIPAAm-grafted PDMS surface were induced into osteoblasts, adipocytes and neurocytes under osteogenic medium, adipogenic medium and neurogenic medium respectively. The PNIPAAm-grafted PDMS surface was favourable for osteogenesis of hMSCs, although the potentials of adipogenesis and neurogenesis of hMSCs on the PNIPAAm-grafted PDMS surface were similar to those on the plate surface coated with gelatin. The above results demonstrate that the PNIPAAm-grafted PDMS surface not only kept the potentials of proliferation and multi-differentiation of hMSCs, but also increased the viability of hMSCs.
Skip Nav Destination
Article navigation
Research Article|
December 15 2009
Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm
Dongyan Shi;
Dongyan Shi
1
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Dan Ma;
Dan Ma
1
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Feiqing Dong;
Feiqing Dong
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Chen Zong;
Chen Zong
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Liyue Liu;
Liyue Liu
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Dan Shen;
Dan Shen
†Laboratory of Bone Marrow, The First Hospital, Zhejiang University, Hangzhou, Zhejiang 310006, China
Search for other works by this author on:
Wenji Yuan;
Wenji Yuan
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Xiangmin Tong;
Xiangmin Tong
‡The Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Hengwu Chen;
Hengwu Chen
‡The Institute of Microanalytical Systems, Department of Chemistry, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
Search for other works by this author on:
Jinfu Wang
Jinfu Wang
2
*The Institute of Cell Biology and Genetics, College of Biological Sciences, Zhejiang University, Zijingang Campus, Hangzhou, Zhejiang 310058, China
1To whom correspondence should be addressed (email [email protected]).
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
February 24 2009
Revision Received:
April 28 2009
Accepted:
May 15 2009
Accepted Manuscript online:
May 15 2009
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2010 Biochemical Society
2010
Biosci Rep (2010) 30 (3): 149–158.
Article history
Received:
February 24 2009
Revision Received:
April 28 2009
Accepted:
May 15 2009
Accepted Manuscript online:
May 15 2009
Connected Content
Citation
Dongyan Shi, Dan Ma, Feiqing Dong, Chen Zong, Liyue Liu, Dan Shen, Wenji Yuan, Xiangmin Tong, Hengwu Chen, Jinfu Wang; Proliferation and multi-differentiation potentials of human mesenchymal stem cells on thermoresponsive PDMS surfaces grafted with PNIPAAm. Biosci Rep 1 June 2010; 30 (3): 149–158. doi: https://doi.org/10.1042/BSR20090026
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |