A dimeric 64 kDa HA (haemagglutinin) was isolated with a high yield from dried Phaseolus vulgaris cultivar ‘French bean number 35’ seeds. It inhibited the proliferation of hepatoma HepG2 cells and breast cancer MCF-7 cells with an IC50 of 100 and 2 μM respectively. After exposure of MCF-7 cells to the HA for 24 h, a number of changes were detected in the cells. Growth arrest in the G0/G1 and G2/M phases was observed. The number of cells undergoing early apoptosis and late apoptosis increased. Disruption of the mitochondrial transmembrane potential and disorganization of the inner mitochondrial membrane were induced. Western-blot analysis disclosed that the HA induced apoptosis through the death receptor-mediated pathway.
Research Article| April 09 2010
First report of a haemagglutinin-induced apoptotic pathway in breast cancer cells
Sze Kwan Lam;
Tzi Bun Ng
Tzi Bun Ng 1
1School of Biomedical Sciences, Faculty of Medicine, The Chinese University of Hong Kong, Shatin, New Territories, Hong Kong, People's Republic of China
1To whom correspondence should be addressed (email email@example.com).
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Sze Kwan Lam, Tzi Bun Ng; First report of a haemagglutinin-induced apoptotic pathway in breast cancer cells. Biosci Rep 1 October 2010; 30 (5): 307–317. doi: https://doi.org/10.1042/BSR20090059
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