CLX (celecoxib), a selective COX-2 (cyclo-oxygenase-2) inhibitor, has numerous pleiotropic effects on the body that may be independent of its COX-2 inhibitory activity. The cancer chemopreventive ability of CLX, particularly in CRC (colorectal cancer), has been shown in epidemiological studies. Here we have, for the first time, examined the biophysical effects of CLX on the cellular membranes of COX-2 expressing (HT29) and COX-2 non-expressing (SW620) cell lines using ATR-FTIR (attenuated total reflectance–Fourier transform IR) spectroscopy and SL-ESR (spin label–ESR) spectroscopy. Our results show that CLX treatment decreased lipid fluidity in the cancer cell lines irrespective of COX-2 expression status. As metastatic cells have higher membrane fluidity, we examined the effect of CLX on the metastatic potential of these cells. The CLX treatment efficiently decreased the proliferation, anchorage-independent growth, ability to close a scratch wound and migration and invasion of the CRC cell lines through Matrigel. We propose that one of the ways by which CLX exerts its anti-tumorigenic effects is via alterations in cellular membrane fluidity which has a notable impact on the cells' metastatic potential.
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September 26 2011
Celecoxib reduces fluidity and decreases metastatic potential of colon cancer cell lines irrespective of COX-2 expression Available to Purchase
Aslı Sade;
Aslı Sade
1Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey
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Seda Tunçay;
Seda Tunçay
1Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey
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İsmail Çimen;
İsmail Çimen
1Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey
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Feride Severcan;
Feride Severcan
1Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey
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Sreeparna Banerjee
Sreeparna Banerjee
1
1Department of Biological Sciences, Middle East Technical University, Ankara 06531, Turkey
1To whom correspondence should be addressed (email [email protected]).
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Publisher: Portland Press Ltd
Received:
December 14 2010
Revision Received:
March 10 2011
Accepted:
March 15 2011
Accepted Manuscript online:
March 15 2011
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© The Authors Journal compilation © 2012 Biochemical Society
2012
Biosci Rep (2012) 32 (1): 35–44.
Article history
Received:
December 14 2010
Revision Received:
March 10 2011
Accepted:
March 15 2011
Accepted Manuscript online:
March 15 2011
Citation
Aslı Sade, Seda Tunçay, İsmail Çimen, Feride Severcan, Sreeparna Banerjee; Celecoxib reduces fluidity and decreases metastatic potential of colon cancer cell lines irrespective of COX-2 expression. Biosci Rep 1 February 2012; 32 (1): 35–44. doi: https://doi.org/10.1042/BSR20100149
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