Hepatic hexose transport was characterized using 3-O-methyl-D-glucose, which is not metabolized by the liver. The kinetic parameters determined in the starved state were taken as basal values for the transport system which showed saturation kinetics with high Vmax and Km values of 161 nmol/mg dry wt./rnin and 39 mM respectively. In the fed state, the Vmax was found to be increased nearly two-fold; this may be due to a phenomenon known as trans-stirnulation. The effects of N2-induced anoxia and of KCN were investigated. In the fasted state, anoxia caused the transport characteristics Vmax and Km to decrease nearly two-fold whereas KCN had the opposite effect as the Vmax and Km were increased by three- and two-fold respectively. In the fed state, anoxia and KCN caused a marked decrease in the transport characteristics.
Skip Nav Destination
Article navigation
Research Article|
October 01 1984
Hepatic hexose transport and the effect of N2-induced anoxia and KCN on this process
G. N. Europe-Finner
G. N. Europe-Finner
1Department of Biochemistry, St. George's Hospital Medical School, Cranmer Terrace, Tooting, London SW17 ORE, UK
*Department of Biochemistry, Oxford University, South Parks Road, Oxford, UK
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
September 06 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (10): 843–849.
Article history
Received:
September 06 1984
Citation
G. N. Europe-Finner; Hepatic hexose transport and the effect of N2-induced anoxia and KCN on this process. Biosci Rep 1 October 1984; 4 (10): 843–849. doi: https://doi.org/10.1007/BF01138166
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |