Measurement of the content of polyamines in pancreatic islets indicated that no significant change in their concentration took place during glucose-stimulated insulin release. The finding, together with the absence of any effect of α-difluoromethylornithine on glucosestimulated insulin release suggested that rapid synthesis of polyamines is not involved in stimulus-secretion coupling in the β-cell. The concentration of polyamines found in islets were high enough for them to act as substrates for the Ca2+-dependent islet transglutaminase during insulin release. This was further demonstrated by the ability of islet transglutaminase to incorporate [14C]putrescine into proteins from islet homogenates and by the demonstration of an increase in the covalent incorporation of [14C]putrescine into the proteins of intact islets following their challenge with glucose. Unlike monoamine substrates of transglutaminase, putrescine failed to effectively inhibit insulin release when its intracellular concentration was increased. A role for polyamines in the secretory process through their incorporation into islet proteins is suggested.
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Research Article|
October 01 1984
A role for polyamines in stimulus-secretion coupling in the pancreatic β-cell
P. J. Bungay;
P. J. Bungay
1Department of Life Sciences, Trent Polytechnic, Clifton Lane, Nottingham NG11 8NS, UK
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J. M. Potter;
J. M. Potter
1Kent and Canterbury Hospital, Canterbury, Kent CT1 3NG, UK
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M. Griffin
M. Griffin
1Department of Life Sciences, Trent Polytechnic, Clifton Lane, Nottingham NG11 8NS, UK
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Publisher: Portland Press Ltd
Received:
September 14 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (10): 869–877.
Article history
Received:
September 14 1984
Citation
P. J. Bungay, J. M. Potter, M. Griffin; A role for polyamines in stimulus-secretion coupling in the pancreatic β-cell. Biosci Rep 1 October 1984; 4 (10): 869–877. doi: https://doi.org/10.1007/BF01138169
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