The nucleotide sequence of the thioredoxin gene from Escherichia coli was determined. The structural gene was identified on a cloned 3-kb PvuII Iragment by hybridization with a synthetic oligodeoxyribonucleotide corresponding to a part of the amino acid sequence of thioredoxin. Restriction-enzyme fragments were used as templates in the dideoxy sequence method, directly and after subcloning into M13mp8. A segment of 450 nucleotides was determined using both strands7 alternatively, without extensive overlaps. The sequence contains the thioredoxin coding region, a potential ribosome-binding site, and a putative promotor region. The predicted amino acid sequence differs by two inversions from the previously given thioredoxin sequence. The revised sequence is presented and the results further show that thioredoxins from E. coli B and K12 are identical.
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November 01 1984
Nucleotide sequence of the thioredoxin gene from Escherichia coli
Jan-Olov Höög;
Jan-Olov Höög
1Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden
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Hedvig von Bahr-Lindström;
Hedvig von Bahr-Lindström
1Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden
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Staffan Josephson;
Staffan Josephson
2Department of Chemistry, KabiGen AB, S-112 87 Stockholm, Sweden
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Betty J. Wallace;
Betty J. Wallace
3Department of Molecular and Population Genetics, University of Georgia, Athens, GA 30602, USA
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Sidney R. Kushner;
Sidney R. Kushner
3Department of Molecular and Population Genetics, University of Georgia, Athens, GA 30602, USA
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Hans Jörnvall;
Hans Jörnvall
1Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden
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Arne Holmgren
Arne Holmgren
1Department of Chemistry I, Karolinska Institutet, S-104 01 Stockholm, Sweden
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Publisher: Portland Press Ltd
Received:
September 27 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (11): 917–923.
Article history
Received:
September 27 1984
Citation
Jan-Olov Höög, Hedvig von Bahr-Lindström, Staffan Josephson, Betty J. Wallace, Sidney R. Kushner, Hans Jörnvall, Arne Holmgren; Nucleotide sequence of the thioredoxin gene from Escherichia coli. Biosci Rep 1 November 1984; 4 (11): 917–923. doi: https://doi.org/10.1007/BF01116889
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