In the presence of 3-isobutyi-l-methylxanthine, VIP produced a dose-related (3 × 10−9 − 10−7 M) increase (g-fold) in cAMP production in isolated HEp-2 cells incubated at 15°C in KRP buffer. Among the peptides structurally related to VIP, including secretin (10−7 M), pancreatic glucagon (10−6 M), PHI, somatostatin-14 (10−6 M), hpGRF (10−8 − 4 × 10−M), GIP (2 × 10−7 M), only PHI (3 × 10−7 M and above) is able to activate the cAMP-generating system in HEp-2 cells, but at 102 times lower potency. Under the same conditions, histamine (10−3 M) was also ineffective, while PGE 2 (10−7 − 10−4 M) increased (0-fold) basal cAMP levels in HEp-2 cells. The VIP effect is related to the interaction os the peptide on VIP recognition sites (12SI-VIP-binding capacity), coupled to the membrane-bound adenylate cyclase. The results indicate that the transformed laryngeal cell line HEp-2 possessesa receptor-cAMP system preferentially activated by VIP (relative potencies: VIP > PHI ≫ other peptides of the secretin family), and suggest that this neuropeptide could modulate biological functions in normal laryngeal epithelia in man.
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December 01 1984
Activation of the cAMP-generating system by vasoactive intestinal polypeptide (VIP) in the human laryngeal malignant cell line HEp-2
Annie Prost;
Annie Prost
1INSERM U. 55, Hôpital St. Antoine, 184 rue du Fg. St. Antoine, 75571 Paris Cedex 12, France
2Service central d'Hématologie (Laboratoire d'Immunologi), Hôpital Tenon, 4 rue de la Chine, 75970 Paris Cedex 20, France
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Shahin Emami;
Shahin Emami
1INSERM U. 55, Hôpital St. Antoine, 184 rue du Fg. St. Antoine, 75571 Paris Cedex 12, France
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Gabriel Rosselin;
Gabriel Rosselin
1INSERM U. 55, Hôpital St. Antoine, 184 rue du Fg. St. Antoine, 75571 Paris Cedex 12, France
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Christian Gespach
Christian Gespach
1INSERM U. 55, Hôpital St. Antoine, 184 rue du Fg. St. Antoine, 75571 Paris Cedex 12, France
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Publisher: Portland Press Ltd
Received:
October 22 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (12): 1045–1050.
Article history
Received:
October 22 1984
Citation
Annie Prost, Shahin Emami, Gabriel Rosselin, Christian Gespach; Activation of the cAMP-generating system by vasoactive intestinal polypeptide (VIP) in the human laryngeal malignant cell line HEp-2. Biosci Rep 1 December 1984; 4 (12): 1045–1050. doi: https://doi.org/10.1007/BF01116697
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