Immunocytochemical localization of 8-hexosaminidase in cultured human skin fibroblasts was performed in the presence or absence of the Na+/K+ ionophores monensin and nigericin. In the presence of monensin, β-hexosaminidase accumulated in the periphery of swollen vesicles in the paranuclear region of fibroblasts from normaI individuals and from patients with mucolipidosis II. Nigericin-treated cells had more extensive vacuolization of the cytoplasm and the localization of the enzyme was more diffuse within these vacuoles. Morphological studies at the ultrastructral level indicated that a perturbation of the Golgi region occurred during ionophore treatment. These findings suggest that β-hexosaminidase in ionophore-treated fibroblasts is trapped in a time- and dose-dependent manner in the paranuclear region presumed to be the swollen cisternae of the Golgi region, or adjacent vesicles derived from the Golgi region. Furthermore, fibrobiasts are more sensitive to perturbation by nigericin than by monensin at similar concentrations and exposure times. These data support biochemical findings that the two ionophores differentially inhibit the transport of lysosomal enzymes in the Golgi region.
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December 01 1984
Immunocytochemical localization on β-hexosaminidase and electron-microscopic characterization of human fibroblasts following treatment with monensin and nigericin
Georgirene D. Vladutiu
Georgirene D. Vladutiu
1Department of Pediatrics, Children's Hospital of Buffalo and State University of New York at Buffalo, 219 Bryant Street, Buffalo, New York 14222, USA
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Publisher: Portland Press Ltd
Received:
September 18 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (12): 1079–1088.
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Received:
September 18 1984
Citation
Georgirene D. Vladutiu; Immunocytochemical localization on β-hexosaminidase and electron-microscopic characterization of human fibroblasts following treatment with monensin and nigericin. Biosci Rep 1 December 1984; 4 (12): 1079–1088. doi: https://doi.org/10.1007/BF01116702
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