The total Na+/K + ATP-ase activity of the thick ascending limb of the loop of Henlé may be stimulated by arginine-vasopressin (AVP). Lysine-vasopressin (LVP), oxytocin (OT), and arginine-vasotocin (AVT) produce less than 5% of the enzyme activity induced by the same concentration of AVP. Physiological concentrations of a mixture of other hormones with known activity on the kidney (T3, T4 aldosterone, angiotensin II, and OT) did not significantly increase total Na+/K + ATP-ase activity. Specific AVP antiserum consistently removed greater than 90% of the stimulatory effect of plasma. The concentration of AVP in plasmas from dehydrated subjects was greater than l0 times that of the same subjects hydrated. Intra-assay coefficient of variation was 35% and 52% from 200 μl and 20 μl of plasma respectively. The interassay coefficient of variation was 53% and 55% from plasma pools with high and low AVP content.
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Research Article|
February 01 1984
Specificity of a ctyochemical bioassay for arginine-vasopressin and its validation for plasma measurement
A. Smith;
A. Smith
1Department of Child Health, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, U.K.
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N. McIntosh
N. McIntosh
1Department of Child Health, St. George's Hospital Medical School, Cranmer Terrace, London SW17 0RE, U.K.
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Publisher: Portland Press Ltd
Received:
December 07 1983
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (2): 109–114.
Article history
Received:
December 07 1983
Citation
A. Smith, N. McIntosh; Specificity of a ctyochemical bioassay for arginine-vasopressin and its validation for plasma measurement. Biosci Rep 1 February 1984; 4 (2): 109–114. doi: https://doi.org/10.1007/BF01120306
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