An enzyme immunoassay, using finely ground rabbit glomerular basement membrane (GBM) as an antigen, was able to detect sheep anti-rabbit GBM antibodies at serum dilutions of 1:32 000. The particulate GBM bound firmly to plastic micro ELISA plates without the aid of a linking agent, and the antigen-coated plates remained stable for several months when stored at −70°C. There were no appreciable differences between amino acid compositions of ground and whole GBM, and no detectable loss of antigens occurred during the grinding procedure. Competitive inhibition assays, with collagenase and pepsin digests of rabbit GBM demonstrated preservation of collagenous and non-collagenous antigens in the ground GBM. The assay should prove to be a relatively simple and highly sensitive technique for detecting antibodies to a wide spectrum of GBM antigens.
Skip Nav Destination
Article navigation
Research Article|
March 01 1984
Measurement of anti-glomerular-basement-membrane antibodies by micro ELISA using insoluble antigens
Kay Barnard;
Kay Barnard
1Department of Pathology, University of Bristol, University Walk, Bristol, UK
*Meat Research Institute, Langford, Bristol BS18 7DY, UK
Search for other works by this author on:
M. P. James
M. P. James
1Department of Pathology, University of Bristol, University Walk, Bristol, UK
**Whangarei Animal Health Laboratory, Ministry of Agriculture & Fisheries, Private Bag, Whangarei, New Zealand
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
January 12 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (3): 195–201.
Article history
Received:
January 12 1984
Citation
Kay Barnard, M. P. James; Measurement of anti-glomerular-basement-membrane antibodies by micro ELISA using insoluble antigens. Biosci Rep 1 March 1984; 4 (3): 195–201. doi: https://doi.org/10.1007/BF01119654
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
2
Views
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |