L6 and L8 rat myoblast cell lines have been selected for resistance to hydroxyurea, an antineoplastic agent whose intracellular target is the rate-limiting enzyme activity of DNA synthesis, ribonucleotide reductase. In contrast to the differentiation-competent parental lines from which they were selected, the drug-resistant lines exhibit a grossly altered or absent myogenic capacity. Independent selections have revealed a strong correlation between changes in ribonucleotide reductase, as determined by velocity levels and product pool analyses, and altered myogenic potential. These results provide the first indication that alterations in this key enzyme activity and its accompanying deoxyribonucleoside triphosphate pools can affect cellular differentiation.
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Research Article|
April 01 1984
Involvement of ribonucleotide reductase in cellular differentiation
David C. Creasey;
David C. Creasey
1Department of Microbiology and Biochemistry, and Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E OV9
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Jim A. Wright
Jim A. Wright
1Department of Microbiology and Biochemistry, and Manitoba Institute of Cell Biology, University of Manitoba, Winnipeg, Manitoba, Canada R3E OV9
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Publisher: Portland Press Ltd
Received:
February 10 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (4): 299–309.
Article history
Received:
February 10 1984
Citation
David C. Creasey, Jim A. Wright; Involvement of ribonucleotide reductase in cellular differentiation. Biosci Rep 1 April 1984; 4 (4): 299–309. doi: https://doi.org/10.1007/BF01140493
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