Marked differences were found in the electrophoretic profiles and amino-acid compositions of components prepared from rat glomerular basement membrane (GBM) by a number of different solubilization procedures. Treatment with reducing agent resulted in a simplified electrophoretic pattern which was characterized by the presence of a major collagenous component with a mol. wt, of 150 000. In contrast, detergent solubilized mainly lower-mol.-wt, material which had a more polar amino-acid composition. When both reagents were used together the majority of the basement-membrane material was so Jubilized within 2 h and components with mol. wts, of 170 000 and 135 000 were predominant in the pro-α region of the gel. Treatment for a further 16 h was required to solubilize higher-mol.-wt, material and to achieve maximum solubility of components in the pro-α region with mol. wts, of 185 000 and 150 000. These methods provide a means of separating subcomponents of rat GBM while avoiding the problems of degradation inherent in enzymatic procedures.
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Research Article|
April 01 1984
Differential solubility of subcomponents of rat glomerular basement membrane
Sarah A. Taylor;
Sarah A. Taylor
1Biochemistry Department, Queen Elizabeth College (University of London), Campden Hill Road, Kensington, London W8 7AH, UK
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Robert G. Price
Robert G. Price
1Biochemistry Department, Queen Elizabeth College (University of London), Campden Hill Road, Kensington, London W8 7AH, UK
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Publisher: Portland Press Ltd
Received:
February 03 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (4): 319–326.
Article history
Received:
February 03 1984
Citation
Sarah A. Taylor, Robert G. Price; Differential solubility of subcomponents of rat glomerular basement membrane. Biosci Rep 1 April 1984; 4 (4): 319–326. doi: https://doi.org/10.1007/BF01140495
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