Human bone cells, maintained in culture, have been subjected to partitioning in an aqueous two-phase system on a countercurrent distribution apparatus. A broad cell distribution was obtained indicating cell-surface heterogeneity. Two major cell populations were identified which appeared to be growing at different rates. The ‘fast’-growing cells had a less hydrophobic cell surface than the ‘slow’-growing cells. Possible relationships of these cell populations with osteoblast differentiation and the potential importance of this technique in studies of osteoblast differentiation are discussed.
Studies of the growth of human bone-derived cells in culture using aqueous two-phase partition
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Paul T. Sharpe, B. R. MacDonald, J. A. Gallagher, T. E. Treffry, R. G. G. Russell; Studies of the growth of human bone-derived cells in culture using aqueous two-phase partition. Biosci Rep 1 May 1984; 4 (5): 415–419. doi: https://doi.org/10.1007/BF01122506
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