Contrary to previous reports, commercially available 1000-nm latex beads were found to be labelable with125I, yielding a product that retained its radiolabel on storage at 4°C and when incubated in tissue-culture media. This finding permitted a radiochemical method to measure phagocytic uptake of latex particles by rat peritoneal macrophages cultured in vitro, and a direct comparison with the established method of particle counting by light microscopy. The two methods yielded closely similar data, demonstrating that the (much more convenient) radiochemical method for quantitating phagocytic uptake is both feasible and reliable. The kinetics of phagocytic uptake of the latex particles and the effect of low temperature and metabolic inhibitors (sodium fluoride and 2,4-dinitrophenol) are described. Ongoing phagocytosis did not alter the rate of fluid-phase pinocytosis by macrophages.
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Research Article|
June 01 1984
Phagocytic uptake of latex beads by rat peritoneal macrophages: Comparison of morphological and radiochemical assay methods
Margaret K. Pratten;
Margaret K. Pratten
1Biochemistry Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffordshire ST5 5BG, U.K.
*Department of Anatomy, University of Leicester, Leicester LE1 7RH, U.K.
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John B. Lloyd
John B. Lloyd
1Biochemistry Research Laboratory, Department of Biological Sciences, University of Keele, Keele, Staffordshire ST5 5BG, U.K.
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Publisher: Portland Press Ltd
Received:
May 16 1984
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1984 The Biochemical Society
1984
Biosci Rep (1984) 4 (6): 497–504.
Article history
Received:
May 16 1984
Citation
Margaret K. Pratten, John B. Lloyd; Phagocytic uptake of latex beads by rat peritoneal macrophages: Comparison of morphological and radiochemical assay methods. Biosci Rep 1 June 1984; 4 (6): 497–504. doi: https://doi.org/10.1007/BF01122225
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