Further studies on the acid-precipitable radioactive substance formed during perfusion of Langendorff rat hearts with [14C]adenosine have shown that very brief (30 s) ischaemia causes a sudden rise (20–35%) in its level in the tissue which is followed by the steady fall we have previously described. Analysis of the products of alkaline hydrolysis of this compound shows that at least 96% of the radioactivity appears in the form of a mixture of 2′- and 3′-AMP as would be expected for RNA while its relatively high resistance to dilute alkali suggests that it is poly A. Subcellular localization studies indicate that radioactivity enters all compartments of the cell, with maximum label in the nucleus. However, a significant proportion is present in the mitochondria and may be poly A acting as the mitochondrial storage form of adenine nucleotides whose existence we have proposed.
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Research Article|
December 01 1985
Further studies on the presumed adenine nucleotide storage compound of rat heart
Peter S. Fitt;
Peter S. Fitt
1Department of Biochemistry, University of Ottawa, Ottawa, Ontario, Canada K1N 9B4
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Borivoj Korecky;
Borivoj Korecky
2Department of Physiology, University of Ottawa, Ottawa, Ontario, Canada K1N 9B4
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Nishi Sharma
Nishi Sharma
1Department of Biochemistry, University of Ottawa, Ottawa, Ontario, Canada K1N 9B4
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Publisher: Portland Press Ltd
Received:
September 26 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (12): 1061–1069.
Article history
Received:
September 26 1985
Citation
Peter S. Fitt, Borivoj Korecky, Nishi Sharma; Further studies on the presumed adenine nucleotide storage compound of rat heart. Biosci Rep 1 December 1985; 5 (12): 1061–1069. doi: https://doi.org/10.1007/BF01119627
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