We have used a human phosphoglycerate kinase-1 (PGK-1) cDNA clone to study expression of PGK-2 during mouse spermatogenesis. Hybrid selection, in vitro translation with product identification by 2-D gel electrophoresis demon-strated that the PGK-1 cDNA clone hybridized to PGK-2 mRNA in mouse testes. Northern analyses of RNA purified from separated spermatogenic cells demonstrated a large increase in abundance of PGK-2 mRNA in post-meiotic cells. Thus, post-meiotic transcription of PGK-2 mRNA is demonstrable with cloned DNA probes.
Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testes
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Robert P. Erickson, Alan M. Michelson, Michael P. Rosenberg, Edwin Sánchez, Stuart H. Orkin; Post-meiotic transcription of phosphoglycerate-kinase 2 in mouse testes. Biosci Rep 1 December 1985; 5 (12): 1087–1091. doi: https://doi.org/10.1007/BF01119630
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