Large granular lymphocytes (LGL) from human blood main-tained in culture for 2 to 6 weeks with IL-2 were found positive in the K562 cell killing assay. The cytoplasmic granules of the LGL were isolated, lysed and the soluble proteins were passed over a Sepharose-anti-C9 column. The retained protein was eluted with NaC1 and found to consist by SDS polyacrylamide gel electrophoresis of essentially one component with a molecular weight of approximately 70 000. The protein did not give a positive precipitation test with anti-human C9 by Ouchterlony analysis, but it reacted reproducibly with anti-human C9 by Western blot analysis. By ELISA the cross reaction with human C9 was less than 1%. The C9 related lymphocyte protein lacked C9 hemolytic activity, but it formed functional pores in liposomes in presence of Ca++. These results suggest that the cytoplasmic granules of human LGL that are capable of killing NK target cells contain C9 related protein which is involved in the cellular cytotoxicity reaction.
Skip Nav Destination
Article navigation
Research Article|
December 01 1985
A C9 related channel forming protein in the cytoplasmic granules of human large granular lymphocytes
Leora S. Zalman;
Leora S. Zalman
1Division of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037, USA
Search for other works by this author on:
Mary A. Brothers;
Mary A. Brothers
1Division of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037, USA
Search for other works by this author on:
Hans J. Müller-Eberhard
Hans J. Müller-Eberhard
1Division of Molecular Immunology, Research Institute of Scripps Clinic, La Jolla, California 92037, USA
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
October 28 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (12): 1093–1100.
Article history
Received:
October 28 1985
Citation
Leora S. Zalman, Mary A. Brothers, Hans J. Müller-Eberhard; A C9 related channel forming protein in the cytoplasmic granules of human large granular lymphocytes. Biosci Rep 1 December 1985; 5 (12): 1093–1100. doi: https://doi.org/10.1007/BF01119631
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |