The effect of experimental cardiac hypertrophy on the enzymes of the malate – aspartate shuttle aspartate aminotransferase (AAT) and malate dehydrogenase (MDH) was studied. (1) Aortic constriction in adult rats resulted in 25% cardiac hypertrophy in 2½–3 weeks. Total DNA (mg per heart) did not change. (2) The proportions of mitochondrial and cytosolic isozymes of AAT and MDH did not change as a result of cardiac hypertrophy. About two-thirds of each enzyme occurred in the mitochondrial form and one-third in the cytosolic form. (3) Total AAT in hypertrophic hearts, in enzyme units per mg DNA, increased by 24% compared to AAT content in the hearts of sham-operated animals. Total MDH did not change. SoIubilized protein increased by 20%. Normal hearts contained 10 times more enzyme units of MDH than of AAT. (4) Cardiac growth stimulation induced in newborn rats did not result in specific changes of either enzyme. It is suggested that true cardiac hypertrophy acts as a specific stimulus for the possibly rate-limiting enzyme AAT of the shuttle.
Skip Nav Destination
Article navigation
Research Article|
February 01 1985
Subcellular distribution of the enzymes of the malate-aspartate shuttle in rat heart and effect of experimental cardiac hypertrophy
Charalampos Mavrides;
Charalampos Mavrides
1Department of Biochemistry and Department of Physiology, University of Ottawa, Ottawa, Ontario, Canada, K1H 8M5
Search for other works by this author on:
Borivoj Korecky
Borivoj Korecky
1Department of Biochemistry and Department of Physiology, University of Ottawa, Ottawa, Ontario, Canada, K1H 8M5
Search for other works by this author on:
Publisher: Portland Press Ltd
Received:
January 08 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (2): 95–100.
Article history
Received:
January 08 1985
Citation
Charalampos Mavrides, Borivoj Korecky; Subcellular distribution of the enzymes of the malate-aspartate shuttle in rat heart and effect of experimental cardiac hypertrophy. Biosci Rep 1 February 1985; 5 (2): 95–100. doi: https://doi.org/10.1007/BF01117055
Download citation file:
Sign in
Don't already have an account? Register
Sign in to your personal account
You could not be signed in. Please check your email address / username and password and try again.
Could not validate captcha. Please try again.
Biochemical Society Member Sign in
Sign InSign in via your Institution
Sign in via your InstitutionGet Access To This Article
Cited By
Open Access for all
We offer compliant routes for all authors from 2025. With library support, there will be no author nor reader charges in 5 journals. Check here |
![]() |