The mitogenic effect of a new growth factor that we recently isolated from the acellular ascitic fluid of the Ehrlich ascites carcinoma grown in vivo was examined. We have called this factor EACF (Ehrlich ascites carcinoma factor). EACF caused initiation of DNA synthesis in the liver, submandibular gland, exorbital lacrimal gland and epithelium of the tongue of adult mice after i.p. injection at a protein concentration of 3 pg per 25 g of body weight. For all tissues examined, except the tongue, EACF initiated DNA synthesis at about 48 to 60 h after injection, with the maximum effect at approx. 85 h, and the stimulatory effect lasting approx. 60 h. The initiation of DNA synthesis in liver, which is normally characterized by only an occasional cell passing through the S phase by EACF is of particular interest. The initiation of DNA synthesis in the liver was not prevented by hypophysectomy. Evidence also indicates that a similar heat-labile growth promoting factor(s) is present in calf serum.
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March 01 1985
Initiation of DNA synthesis in the liver and other tissues of adult mice by a growth factor (EACF) isolated from acellular fluid of the Ehrlich ascites carcinoma
Hsing-Wu Yeh;
Hsing-Wu Yeh
*Department of Biochemistry, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
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E. Robert Burns;
E. Robert Burns
**Department of Anatomy, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
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Yun-Chi Yeh
Yun-Chi Yeh
*Department of Biochemistry, College of Medicine, University of Arkansas for Medical Sciences, Little Rock, Arkansas 72205, USA
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Publisher: Portland Press Ltd
Received:
January 17 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (3): 205–214.
Article history
Received:
January 17 1985
Citation
Hsing-Wu Yeh, E. Robert Burns, Yun-Chi Yeh; Initiation of DNA synthesis in the liver and other tissues of adult mice by a growth factor (EACF) isolated from acellular fluid of the Ehrlich ascites carcinoma. Biosci Rep 1 March 1985; 5 (3): 205–214. doi: https://doi.org/10.1007/BF01119589
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