A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, A comparison of water diffusion in human erythrocytes and ghosts revealed a longer relaxation time in ghosts, corresponding to a decreased exchange rate. However, the diffusional permeability of ghosts was not significantly different from that of erythrocytes. The changes in water diffusion following exposure to p-chloromercuribenzene sulfonate (PCMBS) have been studied on ghosts suspended in isotonic solutions. It was found that a significant inhibitory effect of PCMBS on water diffusion occurred only after several minutes of incubation at 37°C. No inhibition was noticed after short incubation at 0°C as previously used in some labelling experiments. This indicates the location in the membrane interior of the SH groups involved in water diffusion across human erythrocyte membranes. The nuclear magnetic resonance (n. m. r.) method appears as a useful tool for studying changes in water diffusiofl in erythrocyte ghosts with the aim of locating the water channel.
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Research Article|
March 01 1985
Water exchange through erythrocyte membranes: p-choloromercuribenzene sulfonate inhibition of water diffusion in ghosts studied by a nuclear magnetic resonance technique
Gheorghe Benga;
Gheorghe Benga
1Department of Cell Biology, Faculty of Medicine, Medical and Pharmaceutical Institute Cluj-Napoca, 3400 Cluj-Napoca, Roumania
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Octavian Popescu;
Octavian Popescu
1Department of Cell Biology, Faculty of Medicine, Medical and Pharmaceutical Institute Cluj-Napoca, 3400 Cluj-Napoca, Roumania
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Victor I. Pop
Victor I. Pop
1Department of Cell Biology, Faculty of Medicine, Medical and Pharmaceutical Institute Cluj-Napoca, 3400 Cluj-Napoca, Roumania
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Publisher: Portland Press Ltd
Received:
March 03 1985
Online ISSN: 1573-4935
Print ISSN: 0144-8463
© 1985 The Biochemical Society
1985
Biosci Rep (1985) 5 (3): 223–228.
Article history
Received:
March 03 1985
Citation
Gheorghe Benga, Octavian Popescu, Victor I. Pop; Water exchange through erythrocyte membranes: p-choloromercuribenzene sulfonate inhibition of water diffusion in ghosts studied by a nuclear magnetic resonance technique. Biosci Rep 1 March 1985; 5 (3): 223–228. doi: https://doi.org/10.1007/BF01119591
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