Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [ 3H]mannose.
Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled poly-peptide.
Secretion of the non-glycosylated protein is only marginally inhibited.
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© 1985 The Biochemical Society
1985
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