1. Pregnant rabbit mammary gland explants cultured with insulin, prolactin and cortisol synthesize and secrete transferrin radiolabelled with [3H]leucine or [ 3H]mannose.

  2. Addition of tunicamycin inhibits the glycosylation but not synthesis of the [3H]leucine-labelled poly-peptide.

  3. Secretion of the non-glycosylated protein is only marginally inhibited.

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